| 1.1 | Supports coupling of lasers with ≥ 4 wavelengths |
| 1.2 | Laser output modulated via AOTF or direct signal; standby mode extends laser lifespan |
| 1.3 | Laser power suitable for low-light imaging |
| | Wavelength 405 nm Semiconductor Laser, output power ≥ 50 mW |
| | Wavelength 488 nm Semiconductor Laser, output power ≥ 50 mW |
| | Wavelength 594 nm Customized Semiconductor Laser, output power ≥ 50 mW |
| | Wavelength 561 nm Solid-State Laser, output power ≥ 50 mW |
| | Wavelength 640 nm Semiconductor Laser, output power ≥ 40 mW |
| 2. Confocal Module |
| 2.1 | Integrated scanning and detection without fiber optics |
| 2.2 | XY Mirrors ≥ 2; Field of view ≥ 20 mm |
| 2.3 | Scan resolution: 512×512 to 8192×8192 (up to 67.1 million pixels) |
| 2.4 | Pixel integration time: 0.5–10 µs |
| 2.5 | Quick Scan: 3 fps @ 512×512; Standard: 0.88 fps |
| 2.6 | Zoom range: 1–50×, continuously adjustable |
| 2.7 | Fluorescence detector: GaAsP, quantum efficiency ≥ 45% @ 500 nm, ≥ 40% @ 600 nm (photomultiplier tube is built in to the scanning module, non fiber optic connection) |
| 2.8 | 6-hole electric filter wheel, equipped with 4 filters: 460nm/50525nm/50600nm/50 and 690nm/50nm, with a laser cutting efficiency OD ≥ 6 |
| 2.9 | DIC imaging with electric switch; supports overlay analysis |
| 3. Microscope |
| 3.1 | High-eye-point eyepiece PL10X; adjustable binocular head (20°~45°) infinitely far hinged binocular observation head, pupil distance adjustment range: 50~76mm |
| 3.2 | Electric 6-position nosepiece |
| 3.3 | Electric stage (350×248 mm), travel: 114×75 mm, accuracy: 0.5 µm, resolution: 0.02um, maximum speed of stage movement: 50mm/s; Includes 36Petri dish holder, 96 well plate holder, Teski holder; |
| 3.4 | Objectives lens: With a focal length ≤ 45mm, FOV≥ 22mm, RMS international universal thread (GB/T 22055.1-2008), with a focal length compatible with most existing products on the market: Infinite flat field apochromatic objective lens 4X ≥ 0.16, WD≥ 12.8mm; Infinite flat field apochromatic objective with 10X ≥ 0.40 and WD≥3.10mm; Infinite flat field super complex achromatic objective 20X≥ 0.80, WD≥0.60; Infinite flat field super complex achromatic objective 40X≥ 0.95, WD≥0.18mm; Infinite flat field super complex achromatic objective lens 60X≥ 1.42, WD≥ 0.17mm; Infinite flat field super complex achromatic objective 100X≥ 1.45, WD≥0.14mm |
| 3.5 | Spotlight: Seven hole electric spotlight, NA≥0.55，WD≥27mm；Equipped with electric aperture stop, electric turntable, and electric polarizer; Support bright field and contrasting observation. The front of the spotlight has an aperture stop, a dial, and an electric control button for the polarizer |
| 3.6 | DIC available at 10X, 20X, 40X, 60X |
| 3.7 | Research grade inverted rack, low hand position coarse micro coaxial electric focusing mechanism, Z-axis stroke of 10.5mm, focusing accuracy of 10nm. The front digital display screen of the body can display the real-time status of multiple components such as objective magnification, transmission illumination brightness, fluorescence band, output, etc., and switch between fluorescence, phase contrast, and bright field observation modes with one click on the touch screen; |
| 3.8 | The body integrates electric control technology, and the brightness, objective lens, dimming film dial, fluorescence dial, and shutter can be quickly switched through the corresponding buttons on both sides of the panel. The above functions can also be controlled through software operation, making it more convenient for observers to observe under the microscope or capture confocal images. Built in electric upper light port, located on the left side of the body, with spectral ratios of 100:0 and 0:100; Built in electric left light port, with splitting ratios of 0:100, 50:50, 100:0 |
| 3.9 | Double layer optical path, with reserved system function expansion space inside the body, not less than 250X140X65mm. Additional fluorescent turntable, right light port, and stabilizing accessories can be added as needed |
| 3.10 | Tiltable transmissive lighting bracket, transmissive Kola lighting system, adjustable spotlight bracket, travel ≥ 65mm, with 4 sets of color filter installation positions, including LBD color filter/green color filter/frosted glass |
| 3.11 | Laser light source safety management system, supports automatic shutdown of laser when the transmission lighting bracket is tilted in scanning state |
| 3.12 | Long life LED transmission lighting system, with a power of 10W, pre-set filament center, providing a uniform, high contrast, coaxial, constant color temperature, and almost shadow free lighting system |
| 3.13 | Fluorescent device: Electric 8-hole fluorescent turntable, capable of accommodating up to 2 layers and a total of 16 color filter blocks. Equipped with an electric light gate function, the system can determine that the turntable is placed in the upper and lower positions of the rack; B fluorescence filter group: EX480/30, DI505DC, EM535/40, G fluorescence filter group: EX560/40, DI600DC, EM635/60, UV fluorescence filter group: EX375/28, DI415DC, EM460/50, R fluorescence filter group: EX620/50, DI655DC, EM690/50, can add color filter blocks |
| 3.14 | The LED fluorescent light source RFL-4LED contains four excitation channels of 365/460/525/625nm, with channel triggering control<500ms. Independent and controllable light intensity, 10Whigh-power LED, designed for a lifespan of over 20000 hours |
| 3.15 | Electric control box with wide voltage (90–265V AC), CAN bus output |
| 4. Operating Software |
| 4.1 | Research grade software platform for laser tube confocal
microscopy imaging system; Windows 10 compatible |
| 4.2 | Imaging Mode: Supports five dimensional acquisition including x, y, z, λ, t, including various combination imaging modes such as xy λ, xyz λ, xyλt, and xyz λ t; Implement functions such as multi-channel flow acquisition, multi-layer Z-stack acquisition, fluorescence image stitching, and delayed acquisition |
| 4.3 | Microscope control: Manage equipment and configure parameters. Control the device by selecting the observation objective lens, observation mode, supporting optical port selection, Z-axis movement, single step movement, continuous movement, and quick to specified position, and supporting reset. Fluorescence mode selection, light gate switch, spotlight mode selection, polarizer moving in and out and controlling the aperture range, supporting light source brightness control |
| 4.4 | Scan Control: Supports three acquisition modes: standard scan, fast scan, and area scan; Support 0.5-10us pixel integration time adjustment; Support multi-level resolution switching of 512/1024/2048/4096/8192; Support switching between single and bidirectional scanning; Support 1-50xmagnification adjustment; Support frame overlay processing for weak signal sample processing, support averaging processing for filtering noise |
| 4.5 | Stage control: Supports eight directional movement control of electric stage, platform position display, jog step adjustment, stage position memory, recovery and other functions |
| 4.6 | Z-axis control: Focus is achieved by adjusting the Z-axis position of the objective lens converter; Equipped with Z-axis position display, movement, and reset functions, supporting electric step adjustment, XYZ position memory, recovery, and other functions |
| 4.7 | Image acquisition: According to different imaging modes, multi-channel image acquisition is achieved, with each channel independently controlled. Laser and filter blocks can be freely switched, and corresponding laser power, PMT gain, signal offset value, and other information can be set for each channel; Supports image storage in four formats: TIFF, BMP, PNG, and JPG |
| 4.8 | Image information storage: Record confocal acquisition parameters including acquisition mode, imaging mode, objective magnification, pixel integration time, magnification, field of view size, resolution, channel configuration (laser power, PMT gain, offset value), layer scan parameters(starting position, ending position, layer number, layer interval), and image name list |
| 4.9 | Image Preview: A multi-channel preview window that allows for simultaneous preview of multiple acquisition channels, supports real-time synthesis of multiple channels, real-time refreshing of image histograms, and real-time adjustment of image thresholds |
| 4.10 | Image annotation: Preview images support length measurement, optical density measurement, angle measurement, and scale display |
| 4.11 | Image processing: supports 3D reconstruction and display, colocalization processing, co localization linkage, image flipping, mirroring, background removal, dynamic image generation, stack processing, ROI processing; |
| 4.12 | Image analysis: Supports parameter analysis such as perimeter, area, roundness, maximum grayscale, scale bar, minimum grayscale, colocalization analysis, and cell counting |
| 5. Computer |
| CPU | Intel Core i7-13700 or higher |
| RAM | ≥ 32 GB |
| Storage | ≥ 1 TB SSD |
| Graphics Card | NVIDIA RTX 3060 Ti or better |
| Display | 34-inch monitor, 3840×1440 resolution |
| 6. Optical Platform |
| 6.1 | Table size: ≥ 1000 × 800 mm |
| 6.2 | Material: Stainless steel (1Cr17), sub-gloss textured |
| 6.3 | Flatness: 0.05–0.10 mm/㎡ |
| 6.4 | Supports: Four legs with connecting rods |
| 6.5 | Leveling: Mechanical |
| 6.6 | Surface roughness: 0.8–1.6 µm; M6 holes at 25 mm × 25 mm |
| 6.7 | Natural frequency: 10–12 Hz |
