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Hybridization Oven – QualiHO™ Series

Hybridization Oven
Parent Product
Life Science Equipments
Standards
IEC 61010-1
IEC 61326-1
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Hybridization Oven – QualiHO™ Series

A Hybridization Oven provides uniform, temperature-controlled mixing for membrane and tube-based hybridization steps in molecular biology workflows. QualiHO™ Series combines stable heating with rotating motion to keep probes and samples moving evenly across membranes during pre-hybridization, hybridization, and post-hybridization washing.

The platform is designed for repeatable incubation conditions, helping reduce uneven signal, edge effects, and temperature drift across long runs. It supports common hybridization tube formats and simple set-and-run operation for day-to-day lab use.

Hybridization Oven – QualiHO™ Series Applications

  • Pre-hybridization blocking Bring membranes and buffers to a stable temperature while rotating tubes to keep blocking solution evenly distributed before adding probes.
  • DNA/RNA blot hybridization Maintain steady incubation temperature and continuous rotation so probes contact the membrane consistently across the full surface.
  • Membrane washing steps Run controlled wash cycles with timed operation to standardize stringency conditions and reduce between-operator variability.
  • Protocol development and optimization Fine-tune temperature and motion settings when adapting probe concentration, buffer chemistry, or wash conditions for new targets.
Hybridization Oven – QualiHO™ Series

Standards

  • IEC 61010-1 – Safety requirements for electrical equipment for measurement, control, and laboratory use. QualiEP™
  • IEC 61326-1 – EMC requirements (immunity and emissions) for laboratory and measurement equipment.

Hybridization Oven – QualiHO™ Series Key Features

  • Stable temperature control to support consistent hybridization and wash conditions across long incubations.
  • Rotating tube motion (0–30 RPM) for uniform probe exposure and repeatable membrane contact.
  • Fast temperature stabilization with equilibration time under 20 minutes for routine workflows.
  • Timed operation up to 24 hours for unattended runs and standardized protocols.
  • Tube compatibility options to match common tube lengths used in blot hybridization.
  • Vibration option (model-dependent) for added mixing control where protocols benefit from adjustable agitation.

Theory and Method

Hybridization depends on controlled temperature and consistent contact between the probe solution and the target bound to a membrane. Temperature affects binding stringency, while motion prevents stagnant zones that can cause uneven signal. A rotating Hybridization Oven keeps tubes rolling at a set RPM, maintaining mixing without manual handling. Typical workflow includes loading membranes into hybridization tubes, setting temperature and time, running pre-hybridization, adding probe, continuing incubation, then running timed wash steps under controlled temperature.

Hybridization Oven – QualiHO™ Series Technical Specification

SpecificationQualiHO™ 10QualiHO™ 100
Temperature Control RangeRT + 8°C to 100°C
Temperature Control Accuracy±0.5°C
Temperature Display Resolution0.1°C
Temperature Equilibration Time< 20 min
Rotation Speed0–30 RPM
Vibration Intensity—1–10 steps adjustable
Continuous Operating Time1–1440 min (24 hours)
Continuous Operation FunctionYes
Hybridization Tube SpecificationsStandard: Ø35 × 240 mm (2 pcs)Standard: Ø35 × 240 mm (2 pcs)
Optional Tube SizesØ35 × 150 mm; Ø35 × 300 mmØ35 × 150 mm; Ø35 × 300 mm

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Life Science Equipments