Ultrasonic DNA Disruptor

Parent Product

Life Science Equipments

Standards

Ultrasonic DNA Disruptor – QualiDNA™ UD

Ultrasonic DNA Disruptor systems deliver controlled, non-contact sonication for DNA fragmentation and chromatin shearing in sealed tubes. Ultrasonic DNA Disruptor – QualiDNA™ UD is built to process up to 18 samples per run with programmable time, cycle control, and stored methods for repeatable workflows. A closed-container approach helps reduce carryover risk when you handle valuable, low-volume samples.

Labs often use this platform to standardize ChIP and NGS sample prep where fragment size consistency matters. Integrated cooling support is available via a matching recirculating chiller specification in the product documentation, helping limit heat-driven sample damage during longer runs.

Ultrasonic DNA Disruptor – QualiDNA™ UD Applications

DNA fragmentation for NGS library preparation Create a controlled fragment distribution by tuning sonication time and cycle settings. This supports consistent downstream end repair, adapter ligation, and size selection when working across batches.
Chromatin shearing for ChIP workflows Shear crosslinked chromatin to a defined bp window to improve immunoprecipitation efficiency and enrichment consistency. A programmable cycle profile helps repeat conditions across runs and users.
Low-volume, high-value sample processing Run micro-volume samples using supported tube formats and well configurations. This is helpful when sample mass is limited and minimizing loss is a priority.
Multi-sample processing with reduced cross-contamination risk Process multiple closed tubes in one session without a probe touching the sample. This supports cleaner workflows, especially in shared labs or high-throughput schedules.

Standards and compliance

IEC 61010-1 (general safety requirements for laboratory electrical equipment)
UL 61010-1 (U.S. safety standard aligned to IEC 61010-1)
IEC 61326-1 (EMC emissions and immunity for measurement and laboratory equipment)

Ultrasonic DNA Disruptor – QualiDNA™ UD Key features

Non-contact processing in closed tubes to support cleaner workflows and reduce carryover pathways.
Programmable sonication time and cycle counts for repeatable fragmentation profiles across protocols.
High-throughput capacity designed around multi-well tube holders for parallel sample handling.
Defined DNA processing window suitable for common fragmentation targets used in ChIP and sequencing prep.
Method memory to store validated settings and reduce re-entry errors between operators.
Cooling support option with a matching chiller specification for temperature-sensitive runs.

Theory and method

Ultrasonic energy couples into the sample container and generates cavitation in the liquid. Bubble formation and collapse create localized shear forces that break DNA into shorter fragments. Fragment size trends depend on total energy delivered, cycle profile, sample volume, and temperature control.

A non-contact, sealed-tube setup helps limit aerosol formation and reduces the chance of transferring residue between samples. Cooling is commonly used to keep fragmentation conditions stable across the full run window.

Ultrasonic DNA Disruptor – QualiDNA™ UD Technical specification

Item	QualiDNA™ UD
Power	300 W (40–100%)
Ultrasonic time	1–999 s
Number of cycles	1–999
Sample capacity and formats	Standard: 0.2 mL, 12 wells, 5–50 µL; 0.5 mL, 12 wells, 30–150 µL Optional: 1.5–2 mL, 8 wells, 100–800 µL; 5 mL, 8 wells, 500 µL–2 mL
DNA processing range	100–1000 bp
Intelligent storage	20 sets
Voltage	AC 220 V / 50 Hz (110V is also available)
Supported consumables	EP tubes

Optional matching chiller (documented accessory): 0.7 L tank, 300 W cooling, control range 4 °C to ambient, dimensions 240 × 398 × 275 mm, 220 V.

Here are two clean remakes (same content, clearer English). I also standardized terms so it reads like a proper lab report.

Experimental Conditions and Methods

1. DNA sample source

Genomic DNA (gDNA) was prepared from λ phage at a concentration of 20 ng/µL.

2. Ultrasound parameter settings

Power: 100%
Pulsed sonication within the total treatment time: 10 s ON / 10 s OFF, repeated in cycles
Sample tube IDs and total treatment times are listed below.

Sample Setup Table

Tube ID	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15
Total sonication time (min)	4	4	4	5	5	5	7.5	7.5	7.5	10	10	10	15	15	15
Sample volume (µL)	50	50	50	50	50	50	50	50	50	50	50	50	50	50	50
Tube volume (mL)	0.2	0.2	0.2	0.2	0.2	0.2	0.2	0.2	0.2	0.2	0.2	0.2	0.2	0.2	0.2

Ultrasonic DNA Disruptor

Ultrasonic DNA Disruptor – QualiDNA™ UD