UV-VIS Spectrophotometers are key parts of almost every laboratory in the world due to their simplicity and versatility. Qualitest offers a modern, and yet cost-effective range of UV-VIS Spectrophotometers that cover a variety of applications commonly used in basic research, quality control, chemical, pharmaceutical, education, life science, and food and beverage sectors.
Ultraviolet-visible (UV-VIS) spectroscopy is one of the most popular analytical techniques because it is very versatile and able to detect nearly every molecule. With UV-VIS spectroscopy, the UV-VIS light is passed through a sample and the transmittance of light by a sample is measured. From the transmittance (T), the absorbance can be calculated as A=-log (T). An absorbance spectrum is obtained that shows the absorbance of a compound at different wavelengths. The amount of absorbance at any wavelength is due to the chemical structure of the molecule.
UV-VIS can be used in a qualitative manner, to identify functional groups or confirm the identity of a compound by matching the absorbance spectrum. It can also be used in a quantitative manner, as concentration of the analyte is related to the absorbance using Beer's Law. UV-VIS spectroscopy is used to quantify the amount of DNA or protein in a sample, for water analysis, and as a detector for many types of chromatography. Kinetics of chemical reactions are also measured with UV-VIS spectroscopy by taking repeated UV-VIS measurements over time. UV-VIS measurements are generally taken with a spectrophotometer. UV-VIS is also a very popular detector for other analytical techniques, such as chromatography, because it can detect many compounds.
Typically, UV-VIS is not the most sensitive spectroscopy technique, because not a lot of light is absorbed over a short path length. Other spectroscopy techniques such as fluorescence have higher sensitivity, but they are not as generally applicable, as most molecules are not fluorescent. UV-VIS has a similar sensitivity to other absorbance measurements, such as infrared spectroscopy.
Qualitest offers a wide range of single-beam and double-beam UV-VIS Spectrophotometers and more including: